1. Mechanisms how to accumulate various metals in plants
2. Interaction between plant cell wall matrix and metals (metalloides)
3. Signaling mechanisms working in the organ to organ interactions in plants
1. Mechanisms how to accumulate various metals in plants
Our aims- Identification of individual metal accumulation mechanismsUptake at the root, Loading to the xylem vessels, Transport to the shoot, Allocation in the tissue, Localization in the cell, …- Integration of individual metal accumulation mechanismsCommon mechanisms for metal transport, Synchronization of complex mechanisms, …How to study- Metallomic (Ionomic) approachRadioisotopes, Compton Camera, Neutron Activation Analysis, ICP-MS, Atomic Absorption Spectrometry, …- Genomic approachModel plants, Genetically identified resources, …- Experimental plantsArabidopsis thaliana, Lotus japonicus, Populus maximowiczii, …
WAKATSUKI, Sakuya (M2)
Graduate School of Science and Technology
Degree Programs in Life and Earth Sciences
Master's Program in Biology
Analysis of the iron transport mechanisms between organs
in Lotus japonicus
KOMATSUBARA, Kuya (B4)
School of Life and Environmental Sciences
College of Agro-Biological Resource Sciences
Involvement of sodium transporter in
cesium uptake and translocation in rice
2. Interaction between plant cell wall matrix and metals (metalloids)
Our aims- Identification of binding mode of cell wall matrix and metals (metalloids)Apoplast protein and toxic aluminum ion, Cell wall matrix components and silicons, …- Understanding the plant responses in the cell wall to the toxic levels of metalsEnzymatic responses to the aluminum ions, …How to study- Cell wall matrix fractionation approachAnalysis of sugar composition, Differential display, Mass spectrometry, …- Reverse genetic approachT-DNA insertion lines, Gene overexpressions, …- Experimental plantsOryza sativa, Glycine max, Arabidopsis thaliana, …
3. Signaling mechanisms working in the organ to organ interactions in plants
Our aims- Identification of the intercellular messengersMineral deficiency, Dormancy cycle, …
- Understanding the mechanisms how to send and how to receive the intercellular messengers
Ion homeostasis, Intracellular messengers, Transcription factors, …
How to study
- Transcriptomic approach
Model plants, Microarray, Quantitative RT-PCR, …
- Proteomic approach
Differential display, Mass spectrometry, Recombinant protein, …
- Metabolomic approach
Differential display, Mass spectrometry, …
- Experimental plantsArabidopsis thaliana, Lotus japonicus, Populus maximowiczii, …
WAKATSUKI, Sakuya (M2)
Graduate School of Science and Technology
Degree Programs in Life and Earth Sciences
Master's Program in Biology
Analysis of the iron transport mechanisms between organs
in Lotus japonicus