Study of mechanisms how to accumulate various metals in plants
Our aims
- Identification of individual metal accumulation mechanisms
Uptake at the root, Loading to the xylem vessels, Transport to the shoot, Allocation in the tissue, Localization in the cell, …
- Integration of individual metal accumulation mechanisms
Common mechanisms for metal transport, Synchronization of complex mechanisms, …
How to study
- Metallomic (Ionomic) approach
Radioisotopes, Neutron Activation Analysis, ICP-AES, Atomic Absorption Spectrometry, …
- Genomic approach
Model plants, Genetically identified resources, …
- Experimental plants
Arabidopsis thaliana, Lotus japonicus, Populus maximowiczii, …


Keita MATSUOKA
Graduate School of
Life and Environmental Sciences
Regulation of gene expressions related to iron-uptake mechanisms in Arabidopsis thaliana

Yoshiaki IWATA
Graduate School of
Life and Environmental Sciences
Metallomics in model legume Lotus japonicus

Masafumi KANAZAWA
Graduate School of
Life and Environmental Sciences
Environmental regulation of metal transport in Poplar



Study of interaction between plant cell wall matrix and metals (metalloids)
Our aims
- Identification of binding mode of cell wall matrix and metals (metalloids)
Apoplast protein and toxic aluminum ion, Cell wall matrix components and silicons, …
- Understanding the plant responses in the cell wall to the toxic levels of metals
Enzymatic responses to the aluminum ions, …
How to study
- Cell wall matrix fractionation approach
Analysis of sugar composition, Difference display, Mass spectrometry, …
- Reverse genetic approach
T-DNA insertion lines, Gene overexpressions, …
- Experimental plants
Oryza sativa, Glycine max, Arabidopsis thaliana, …


Tsuyoshi YAMAMOTO
Graduate School of
Life and Environmental Sciences
Interaction between rice cell wall matrix component and silicon

Eri SOGA
Graduate School of
Life and Environmental Sciences
Identification of cell wall matrix protein related to aluminum toxicity and tolerance

　　



Identification of the signaling mechanisms working in the organ to organ interactions in plants
Our aims
- Identification of the intercellular messengers

　　　　　　　Mineral deficiency, Dormancy cycle, …

　　　　- Understanding the mechanisms how to send and how to receive the intercellular messengers

　　　　　　　Ion homeostasis, Intracellular messengers, Transcription factors, …

How to study

　　　　- Transcriptomic approach

　　　　　　　Model plants, Microarray, Quantitative RT-PCR, …

　　　　- Proteomic approach

　　　　　　　Difference display, Mass spectrometry, Recombinant protein, …

　　　　- Metabolomic approach

　　　　　　　Difference display, Mass spectrometry, …

- Experimental plants
　　　 Arabidopsis thaliana, Populus maximowiczii, …


Keita MATSUOKA
Graduate School of
Life and Environmental Sciences
Regulation of gene expressions related to iron-uptake mechanisms in Arabidopsis thaliana

Masafumi KANAZAWA
Graduate School of
Life and Environmental Sciences
Environmental regulation of metal transport in Poplar


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